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upright fluorescence microscope nikon 90i  (Nikon)


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    Structured Review

    Nikon upright fluorescence microscope nikon 90i
    Upright Fluorescence Microscope Nikon 90i, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/90i+upright+fluorescence+microscope/pmc12276188-185-20-19
    Average 90 stars, based on 1 article reviews
    upright fluorescence microscope nikon 90i - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Staining:

    Article Title: The industrial solvent trichloroethylene induces LRRK2 kinase activity and dopaminergic neurodegeneration in a rat model of Parkinson’s disease
    Article Snippet: .. Briefly, nigral tissue sections were stained for TH and counterstained with DAPI and Nissl NeuroTrace Dye (640; Life Technologies) then imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Article Title: Protection from α-Synuclein induced dopaminergic neurodegeneration by overexpression of the mitochondrial import receptor TOM20
    Article Snippet: .. Briefly, 35 μm coronal nigral tissue sections (1/6 sampling fraction encompassing the volume of the entire SN) were stained for TH and counterstained with DAPI and NeuroTrace Dye (640; Life Technologies) and imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Article Title: Sex Differences in Rotenone Sensitivity Reflect the Male-to-Female Ratio in Human Parkinson’s Disease Incidence
    Article Snippet: .. Briefly, nigral tissue sections were stained for TH and counterstained with 4′,6-diamidino-2-phenylindole, nuclear marker (DAPI) and NeuroTrace Dye (640; Life Technologies) and imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Article Title: Platinum chemotherapeutic-induced oxidative stress affects the transcriptional response of DNA repair genes in murine mesenchymal stem cells
    Article Snippet: .. 10 μl of the stained mixture was placed on a slide and examined using a Nikon 90i upright fluorescence microscope (Nikon, Japan). ..

    Article Title: The industrial solvent trichloroethylene induces LRRK2 kinase activity and dopaminergic neurodegeneration in a rat model of Parkinson’s disease
    Article Snippet: .. Briefly, nigral tissue sections were stained for TH and counterstained with DAPI and Nissl NeuroTrace Dye (640; Life Technologies) then imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Fluorescence:

    Article Title: The industrial solvent trichloroethylene induces LRRK2 kinase activity and dopaminergic neurodegeneration in a rat model of Parkinson’s disease
    Article Snippet: .. Briefly, nigral tissue sections were stained for TH and counterstained with DAPI and Nissl NeuroTrace Dye (640; Life Technologies) then imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Article Title: Protection from α-Synuclein induced dopaminergic neurodegeneration by overexpression of the mitochondrial import receptor TOM20
    Article Snippet: .. Briefly, 35 μm coronal nigral tissue sections (1/6 sampling fraction encompassing the volume of the entire SN) were stained for TH and counterstained with DAPI and NeuroTrace Dye (640; Life Technologies) and imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Article Title: Sex Differences in Rotenone Sensitivity Reflect the Male-to-Female Ratio in Human Parkinson’s Disease Incidence
    Article Snippet: .. Briefly, nigral tissue sections were stained for TH and counterstained with 4′,6-diamidino-2-phenylindole, nuclear marker (DAPI) and NeuroTrace Dye (640; Life Technologies) and imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Article Title: Platinum chemotherapeutic-induced oxidative stress affects the transcriptional response of DNA repair genes in murine mesenchymal stem cells
    Article Snippet: .. 10 μl of the stained mixture was placed on a slide and examined using a Nikon 90i upright fluorescence microscope (Nikon, Japan). ..

    Article Title: VGLUT2 Is a Determinant of Dopamine Neuron Resilience in a Rotenone Model of Dopamine Neurodegeneration
    Article Snippet: Briefly, nigral tissue sections were stained for TH and counterstained with DAPI, since NeuN is not a reliable marker of DA neurons (Cannon and Greenamyre, 2009), and fluorescent Nissl NeuroTrace Dye (640; Thermo Fisher Scientific, Invitrogen). .. Sections were imaged on a Nikon 90i upright fluorescence microscope equipped with high NA plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Collected images were then processed using Nikon NIS-Elements Advanced Research software (version 4.5, Nikon), which enabled unbiased, automated quantitation of colocalizing DAPI, TH, and Nissl-positive stains.

    Article Title: The industrial solvent trichloroethylene induces LRRK2 kinase activity and dopaminergic neurodegeneration in a rat model of Parkinson’s disease
    Article Snippet: .. Briefly, nigral tissue sections were stained for TH and counterstained with DAPI and Nissl NeuroTrace Dye (640; Life Technologies) then imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Microscopy:

    Article Title: The industrial solvent trichloroethylene induces LRRK2 kinase activity and dopaminergic neurodegeneration in a rat model of Parkinson’s disease
    Article Snippet: .. Briefly, nigral tissue sections were stained for TH and counterstained with DAPI and Nissl NeuroTrace Dye (640; Life Technologies) then imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Article Title: Protection from α-Synuclein induced dopaminergic neurodegeneration by overexpression of the mitochondrial import receptor TOM20
    Article Snippet: .. Briefly, 35 μm coronal nigral tissue sections (1/6 sampling fraction encompassing the volume of the entire SN) were stained for TH and counterstained with DAPI and NeuroTrace Dye (640; Life Technologies) and imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Article Title: Sex Differences in Rotenone Sensitivity Reflect the Male-to-Female Ratio in Human Parkinson’s Disease Incidence
    Article Snippet: .. Briefly, nigral tissue sections were stained for TH and counterstained with 4′,6-diamidino-2-phenylindole, nuclear marker (DAPI) and NeuroTrace Dye (640; Life Technologies) and imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Article Title: Platinum chemotherapeutic-induced oxidative stress affects the transcriptional response of DNA repair genes in murine mesenchymal stem cells
    Article Snippet: .. 10 μl of the stained mixture was placed on a slide and examined using a Nikon 90i upright fluorescence microscope (Nikon, Japan). ..

    Article Title: VGLUT2 Is a Determinant of Dopamine Neuron Resilience in a Rotenone Model of Dopamine Neurodegeneration
    Article Snippet: Briefly, nigral tissue sections were stained for TH and counterstained with DAPI, since NeuN is not a reliable marker of DA neurons (Cannon and Greenamyre, 2009), and fluorescent Nissl NeuroTrace Dye (640; Thermo Fisher Scientific, Invitrogen). .. Sections were imaged on a Nikon 90i upright fluorescence microscope equipped with high NA plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Collected images were then processed using Nikon NIS-Elements Advanced Research software (version 4.5, Nikon), which enabled unbiased, automated quantitation of colocalizing DAPI, TH, and Nissl-positive stains.

    Article Title: The industrial solvent trichloroethylene induces LRRK2 kinase activity and dopaminergic neurodegeneration in a rat model of Parkinson’s disease
    Article Snippet: .. Briefly, nigral tissue sections were stained for TH and counterstained with DAPI and Nissl NeuroTrace Dye (640; Life Technologies) then imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Imaging:

    Article Title: The industrial solvent trichloroethylene induces LRRK2 kinase activity and dopaminergic neurodegeneration in a rat model of Parkinson’s disease
    Article Snippet: .. Briefly, nigral tissue sections were stained for TH and counterstained with DAPI and Nissl NeuroTrace Dye (640; Life Technologies) then imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Article Title: Protection from α-Synuclein induced dopaminergic neurodegeneration by overexpression of the mitochondrial import receptor TOM20
    Article Snippet: .. Briefly, 35 μm coronal nigral tissue sections (1/6 sampling fraction encompassing the volume of the entire SN) were stained for TH and counterstained with DAPI and NeuroTrace Dye (640; Life Technologies) and imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Article Title: Sex Differences in Rotenone Sensitivity Reflect the Male-to-Female Ratio in Human Parkinson’s Disease Incidence
    Article Snippet: .. Briefly, nigral tissue sections were stained for TH and counterstained with 4′,6-diamidino-2-phenylindole, nuclear marker (DAPI) and NeuroTrace Dye (640; Life Technologies) and imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Article Title: VGLUT2 Is a Determinant of Dopamine Neuron Resilience in a Rotenone Model of Dopamine Neurodegeneration
    Article Snippet: Briefly, nigral tissue sections were stained for TH and counterstained with DAPI, since NeuN is not a reliable marker of DA neurons (Cannon and Greenamyre, 2009), and fluorescent Nissl NeuroTrace Dye (640; Thermo Fisher Scientific, Invitrogen). .. Sections were imaged on a Nikon 90i upright fluorescence microscope equipped with high NA plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Collected images were then processed using Nikon NIS-Elements Advanced Research software (version 4.5, Nikon), which enabled unbiased, automated quantitation of colocalizing DAPI, TH, and Nissl-positive stains.

    Article Title: The industrial solvent trichloroethylene induces LRRK2 kinase activity and dopaminergic neurodegeneration in a rat model of Parkinson’s disease
    Article Snippet: .. Briefly, nigral tissue sections were stained for TH and counterstained with DAPI and Nissl NeuroTrace Dye (640; Life Technologies) then imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Sampling:

    Article Title: Protection from α-Synuclein induced dopaminergic neurodegeneration by overexpression of the mitochondrial import receptor TOM20
    Article Snippet: .. Briefly, 35 μm coronal nigral tissue sections (1/6 sampling fraction encompassing the volume of the entire SN) were stained for TH and counterstained with DAPI and NeuroTrace Dye (640; Life Technologies) and imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.

    Marker:

    Article Title: Sex Differences in Rotenone Sensitivity Reflect the Male-to-Female Ratio in Human Parkinson’s Disease Incidence
    Article Snippet: .. Briefly, nigral tissue sections were stained for TH and counterstained with 4′,6-diamidino-2-phenylindole, nuclear marker (DAPI) and NeuroTrace Dye (640; Life Technologies) and imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). .. Images were processed using Nikon NIS-Elements Advanced Research software (Version 4.5, Nikon, Melville, NY), and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.



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    Patients who are vsnCTC+ have CTCs with decreased EMD expression. A, EMD expression in vsnCTCs by a <t>fluorescence</t> <t>microscope.</t> DAPI (blue), EMD (orange), CK (green), and CD45 (red) staining is shown (scale bar, 10 μm). The vsnCTCs (<8.5 μm, DAPI+/CK+/CD45 − ) were stained with EMD. Arrows, EMD-positive punctate structures. B, EMD expression in individual WBCs, non-vsnCTCs, and vsnCTCs. C, Spearman correlation between EMD expression and the nucleus size of the CTC in patients with mCRPC. D, Spearman correlation between CK expression and the nucleus size of the CTC in patients with mCRPC. *, P < 0.05; ***, P < 0.001; ns, nonsignificant.
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    Image Search Results


    Patients who are vsnCTC+ have CTCs with decreased EMD expression. A, EMD expression in vsnCTCs by a fluorescence microscope. DAPI (blue), EMD (orange), CK (green), and CD45 (red) staining is shown (scale bar, 10 μm). The vsnCTCs (<8.5 μm, DAPI+/CK+/CD45 − ) were stained with EMD. Arrows, EMD-positive punctate structures. B, EMD expression in individual WBCs, non-vsnCTCs, and vsnCTCs. C, Spearman correlation between EMD expression and the nucleus size of the CTC in patients with mCRPC. D, Spearman correlation between CK expression and the nucleus size of the CTC in patients with mCRPC. *, P < 0.05; ***, P < 0.001; ns, nonsignificant.

    Journal: Clinical Cancer Research

    Article Title: Emerin Dysregulation Drives the Very-Small-Nuclear Phenotype and Lineage Plasticity That Associate with a Clinically Aggressive Subtype of Prostate Cancer

    doi: 10.1158/1078-0432.CCR-24-3660

    Figure Lengend Snippet: Patients who are vsnCTC+ have CTCs with decreased EMD expression. A, EMD expression in vsnCTCs by a fluorescence microscope. DAPI (blue), EMD (orange), CK (green), and CD45 (red) staining is shown (scale bar, 10 μm). The vsnCTCs (<8.5 μm, DAPI+/CK+/CD45 − ) were stained with EMD. Arrows, EMD-positive punctate structures. B, EMD expression in individual WBCs, non-vsnCTCs, and vsnCTCs. C, Spearman correlation between EMD expression and the nucleus size of the CTC in patients with mCRPC. D, Spearman correlation between CK expression and the nucleus size of the CTC in patients with mCRPC. *, P < 0.05; ***, P < 0.001; ns, nonsignificant.

    Article Snippet: Candidate CTCs were characterized as 4′,6-diamidino-2-phenylindole (DAPI)–positive/cytokeratin (CK)-positive/CD45-negative cells with round or oval nuclei using an upright fluorescence microscope (Eclipse 90i, Nikon Instruments Inc.).

    Techniques: Expressing, Fluorescence, Microscopy, Staining